Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice are viable and fertile. Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice are viable and fertile. Hodaka FUJII true The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 190, 5506-5515 (2013). RBRC05950 GARP (Lrrc32) 遺伝子のfloxedマウス。制御性T細胞等に発現しているGARP遺伝子のシグナルペプチドをコードするエクソン1の両側にloxPサイトを持つ。全身でGARP遺伝子を欠損させると周産期致死。T細胞特異的に欠損させると、軽微な免疫学的異常が観察されている。 大阪大学微生物病研究所・藤井穂高先生（2009）。(C57BL/6N x 129S6/SvEvTac)F1由来のiTL BA1 ES細胞を用いて作出。 藤井　穂高 C（3〜6か月） FLP/frt system 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Immunol., 190, 5506-5515 (2013). Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> GARP (Lrrc32) gene floxed mice. Exon 1 of the Lrrc32 gene was flanked by loxP sites. Cre/loxP system GARP (Lrrc32) 遺伝子のfloxedマウス。制御性T細胞等に発現しているGARP遺伝子のシグナルペプチドをコードするエクソン1の両側にloxPサイトを持つ。全身でGARP遺伝子を欠損させると周産期致死。T細胞特異的に欠損させると、軽微な免疫学的異常が観察されている。, Phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse GARP (LRRC32) genomic DNA iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1] <a href='https://brc.riken.jp/mus/pcr05950'>Genotyping protocol -PCR-</a> Developed by Hodaka Fujii, Research Institute for Microbial Diseases, Osaka University in 2009. iTL BA1 ES Cells derived from (C57BL/6N x 129S6/SvEvTac)F1 were used. The mutant mice were crossed to C57BL/6. B6.Cg-Lrrc32<tm1Hfuj> B6.Cg-Lrrc32<tm1Hfuj> C (3-6 months) GARP-Floxed, B6-Lrrc32 floxed GARP-Floxed, B6-Lrrc32 floxed